Mycoplasma chemically extracted from broth culture chemically inactivated to eliminate infectivity. 

Gram-negative pleomorphic cells, spherical to ovoid shaped, single tripled-layered membrane but lacking true cell wall. Causes lower respiratory infections; less often a pharyngitis progressing to bronchitis or pneumoniae; gradual onset with headache, malaise, paroxysmal cough, substernal pain; leucocytosis in some cases. Duration varies from a few days to a month or more. Low fatality.

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Inactivation & Safety — Mycoplasma is inactivated using a chemical process and this inactivation verified by attempting to culture the antigen under ideal conditions.

Applications — ELISA, Latex assays, Western blot.